Isotopic Tracer Elements and Stereochemistry

Mechanism of rhodanese action: isotopic tracer studies.

In a previous publication (2) a double displacement mechanism for the catalytic activity of rhodanese (thiosulfate cyanide sulfurtransferase) was proposed on the basis of polarographic evidence. In addition it was concluded from the polarographic data that crystahine beef liver rhodanese is an enzyme-substrate intermediate containing two activated substrate sulfur atoms per molecule. This commu...

Fructose metabolism in humans – what isotopic tracer studies tell us

Fructose consumption and its implications on public health are currently under study. This work reviewed the metabolic fate of dietary fructose based on isotope tracer studies in humans. The mean oxidation rate of dietary fructose was 45.0% ± 10.7 (mean ± SD) in non-exercising subjects within 3-6 hours and 45.8% ± 7.3 in exercising subjects within 2-3 hours. When fructose was ingested together ...

The Use of Tracer Elements in Biology

Isotopic tracer studies on the biochemistry of cancer.

Chemical studies on cancer have commonly been stimulated by the belief that there exists some distinct biochemical difference between neo plastic and normal cells which, when found, would at once serve to explain the mechanism of the neo plastic transformation and to provide a rational approach to the eradication of cancer. The outstanding example of this type of think ing is Warburg's observat...

Isotopic Tracer Studies on the Movement Of’ Water and Ions between Intestinal Lumen and Blood1

I. STATEMENT OF PROBLEM. The mechanism of movement of matcGJs across biological membranes such as the intestina,l epithelium has rccckcd much attention but many aspects of the problems involved arc still obscure. Studies have been made, for the most part, of net movement of makrial out of or into the gut. However, the intestinal cpithclium is a structure at’ross which materials normally move in...